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NF90/ILF3 Monoclonal antibody, PBS Only (Capture)

NF90/ILF3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10G3

Cat no : 68213-1-PBS

Synonyms

ILF3, IL-F3, DRBP76, DRBF, CBTF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NF90/ILF3 Monoclonal antibody (68213-1-Ig)

    WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NF90/ILF3 Monoclonal antibody (68213-1-Ig)

    WB analysis using 68213-1-Ig (same clone as 68213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using NF90/ILF3 Monoclonal antibody (68213-1-Ig)

    IHC staining of mouse eye using 68213-1-Ig (same clone as 68213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68213-1-Ig (NF90/ILF3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68213-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50472-3

    Cytometric bead array standard curve of MP50472-3

    Cytometric bead array standard curve of MP50472-3, NF90,ILF3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68213-1-PBS. Detection antibody: 68213-4-PBS. Standard:Ag30868. Range: 0.781-100 ng/mL.

Product Information

68213-1-PBS targets NF90/ILF3 as part of a matched antibody pair:

MP50472-3: 68213-1-PBS capture and 68213-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF90/ILF3 fusion protein Ag30868
Full Name interleukin enhancer binding factor 3, 90kDa
Calculated Molecular Weight 894 aa, 95 kDa
Observed Molecular Weight90 kDa, 110 kDa
GenBank Accession NumberBC064836
Gene Symbol ILF3
Gene ID (NCBI) 3609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.