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SEPT6 Monoclonal antibody

SEPT6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1G1

Cat no : 68173-1-Ig

Synonyms

KIAA0128, SEP2, SEPT2, SEPT6, septin 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEPT6 Monoclonal antibody (68173-1-Ig)

    WB analysis using 68173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68173-1-Ig (SEPT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEPT6 Monoclonal antibody (68173-1-Ig)

    IF Staining of HEK-293 using 68173-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody (68173-1-Ig, Clone: 1B1G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Pig brain tissue, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68173-1-Ig targets SEPT6 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Reactivity Human, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SEPT6 fusion protein Ag32736
Full Name septin 6
Calculated Molecular Weight 434 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC036240
Gene Symbol SEPT6
Gene ID (NCBI) 23157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.

Protocols

Product Specific Protocols
WB protocol for SEPT6 antibody 68173-1-IgDownload protocol
IF protocol for SEPT6 antibody 68173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols