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DDAH2 Monoclonal antibody, PBS Only (Detector)

DDAH2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10G3

Cat no : 68154-1-PBS

Synonyms

Dimethylargininase 2, DDAHII, DDAH-2, DDAH 2, DDAH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis using 68154-1-Ig (same clone as 68154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse retina tissue using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis of mouse retina using 68154-1-Ig (same clone as 68154-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis of rat colon using 68154-1-Ig (same clone as 68154-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68154-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50417-2

    Cytometric bead array standard curve of MP50417-2

    Cytometric bead array standard curve of MP50417-2, DDAH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68154-4-PBS. Detection antibody: 68154-1-PBS. Standard:Ag7127. Range: 0.391-50 ng/mL.

Product Information

68154-1-PBS targets DDAH2 as part of a matched antibody pair:

MP50417-2: 68154-4-PBS capture and 68154-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen DDAH2 fusion protein Ag7127
Full Name dimethylarginine dimethylaminohydrolase 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001435
Gene Symbol DDAH2
Gene ID (NCBI) 23564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.