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DDAH2 Monoclonal antibody

DDAH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Rat, Mouse, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A10G3

Cat no : 68154-1-Ig

Synonyms

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis using 68154-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse retina tissue using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis of mouse retina using 68154-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using DDAH2 Monoclonal antibody (68154-1-Ig)

    WB analysis of rat colon using 68154-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inrabbit brain tissue, mouse retina tissue, rat colon tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68154-1-Ig targets DDAH2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit samples.

Tested Reactivity Human, Rat, Mouse, Rabbit
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen DDAH2 fusion protein Ag7127
Full Name dimethylarginine dimethylaminohydrolase 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001435
Gene Symbol DDAH2
Gene ID (NCBI) 23564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.

Protocols

Product Specific Protocols
WB protocol for DDAH2 antibody 68154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols