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Dystrophin Monoclonal antibody

Dystrophin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G12E6

Cat no : 68120-1-Ig

Synonyms

DMD, BMD, 1G12E6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dystrophin Monoclonal antibody (68120-1-Ig)

    WB analysis using 68120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Dystrophin Monoclonal antibody (68120-1-Ig)

    WB analysis of HeLa using 68120-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using Dystrophin Monoclonal antibody (68120-1-Ig)

    WB analysis of rat heart using 68120-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68120-1-Ig (Dystrophin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Dystrophin Monoclonal antibody (68120-1-Ig)

    WB analysis using 68120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68120-1-Ig (DMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using Dystrophin Monoclonal antibody (68120-1-Ig)

    IHC staining of rat skeletal muscle using 68120-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Dystrophin Monoclonal antibody (68120-1-Ig)

    IHC staining of mouse skeletal muscle using 68120-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68120-1-Ig (Dystrophin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Dystrophin Monoclonal antibody (68120-1-Ig)

    IF Staining of mouse skeletal muscle using 68120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using Dystrophin Monoclonal antibody (68120-1-Ig)

    IF Staining of rat heart using 68120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (68120-1-Ig, Clone: 1G12E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Dystrophin Monoclonal antibody (68120-1-Ig)

    FC experiment of HepG2 using 68120-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Dystrophin (68120-1-Ig, Clone:1G12E6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrat brain tissue, HeLa cells, rat heart tissue, HepG2 cells, mouse brain tissue
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue, rat heart tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68120-1-Ig targets Dystrophin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Dystrophin fusion protein Ag4392
Full Name dystrophin
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight70 kDa, 430 kDa
GenBank Accession NumberBC028720
Gene Symbol Dystrophin
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Protocols

Product Specific Protocols
WB protocol for Dystrophin antibody 68120-1-IgDownload protocol
IHC protocol for Dystrophin antibody 68120-1-IgDownload protocol
IF protocol for Dystrophin antibody 68120-1-IgDownload protocol
FC protocol for Dystrophin antibody 68120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols