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SERPINB9 Monoclonal antibody

SERPINB9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9D2

Cat no : 67950-1-Ig

Synonyms

CAP 3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI 9, PI9, Serpin B9, SERPINB9

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of K-562 using 67950-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Daudi using 67950-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Raji using 67950-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of Ramos using 67950-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    WB analysis of human placenta using 67950-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67950-1-Ig (SERPINB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IHC staining of mouse lung using 67950-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IHC staining of mouse lung using 67950-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67950-1-Ig (SERPINB9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using SERPINB9 Monoclonal antibody (67950-1-Ig)

    IF Staining of C6 using 67950-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SERPINB9 antibody (67950-1-Ig, Clone: 2H9D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, human placenta tissue, Daudi cells, Raji cells, Ramos cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67950-1-Ig targets SERPINB9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERPINB9 fusion protein Ag6719
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 9
Calculated Molecular Weight 42 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC002538
Gene Symbol SERPINB9
Gene ID (NCBI) 5272
RRIDAB_2918702
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.

Protocols

Product Specific Protocols
WB protocol for SERPINB9 antibody 67950-1-IgDownload protocol
IHC protocol for SERPINB9 antibody 67950-1-IgDownload protocol
IF protocol for SERPINB9 antibody 67950-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols