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WIF1 Monoclonal antibody

WIF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G6A3

Cat no : 67904-1-Ig

Synonyms

WIF 1, WIF1, WNT inhibitory factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WIF1 Monoclonal antibody (67904-1-Ig)

    WB analysis using 67904-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67904-1-Ig (WIF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inCaco-2 cells, SW480 cells, COLO 320 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67904-1-Ig targets WIF1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WIF1 fusion protein Ag23972
Full Name WNT inhibitory factor 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC018037
Gene Symbol WIF1
Gene ID (NCBI) 11197
RRIDAB_2918660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for WIF1 antibody 67904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bone

Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway

Authors - Yuanxi Leng
View Article
humanWB

Aging (Albany NY)

Metastasis-related gene signature associates with immunity and predicts prognosis accurately in patients with osteosarcoma

Authors - Sen Qin
View Article