• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4A4C9

Cat no : 67902-1-Ig

Synonyms

RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of mouse brain tissue using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis of mouse brain using 67902-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis of rat cerebellum using 67902-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RAB11A Monoclonal antibody (67902-1-Ig)

    WB analysis of rat brain using 67902-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAB11A Monoclonal antibody (67902-1-Ig)

    IF Staining of HeLa using 67902-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using RAB11A Monoclonal antibody (67902-1-Ig)

    FC experiment of HeLa using 67902-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, A549 cells, HeLa cells, pig brain tissue, rat brain tissue, rat cerebellum tissue, HepG2 cells, K-562 cells, rabbit brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67902-1-Ig targets RAB11A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB11A fusion protein Ag8879
Full Name RAB11A, member RAS oncogene family
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2918658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

Protocols

Product Specific Protocols
WB protocol for RAB11A antibody 67902-1-IgDownload protocol
IF protocol for RAB11A antibody 67902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

X (Verified Customer) (10-17-2024)

A very good antibody to detect endo Rab11A from several sources.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
RAB11A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:67902-1-Ig)