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HBB Monoclonal antibody

HBB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11E3

Cat no : 67899-1-Ig

Synonyms

beta globin, CD113t C, HBB, Hemoglobin beta chain, Hemoglobin subunit beta, hemoglobin, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBB Monoclonal antibody (67899-1-Ig)

    WB analysis using 67899-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67899-1-Ig (HBB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman testis tissue, human placenta, human peripheral blood platelets, human plasma, rabbit brain, rabbit liver

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67899-1-Ig targets HBB in WB, ELISA applications and shows reactivity with Human, rabbit samples.

Tested Reactivity Human, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HBB fusion protein Ag9216
Full Name hemoglobin, beta
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight14-16 Da
GenBank Accession NumberBC007075
Gene Symbol HBB
Gene ID (NCBI) 3043
RRIDAB_2918655
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias.

Protocols

Product Specific Protocols
WB protocol for HBB antibody 67899-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols