HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 67886-1-Ig
K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 67886-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67886-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
FC experiment of SW 1990 using 67886-1-Ig
1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 67886-1-Ig
K-562 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 67886-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 67886-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of SW 1990 using 67886-1-Ig
1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KDM1B Monoclonal antibody
Catalog Number
67886-1-Ig
Citations
-
Dilutions
WB : 1:1000-1:6000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
