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CD42b Monoclonal antibody

CD42b Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G1F5

Cat no : 67857-1-Ig

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD42b Monoclonal antibody (67857-1-Ig)

    WB analysis of human peripheral blood platelets using 67857-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human blood using CD42b Monoclonal antibody (67857-1-Ig)

    WB analysis of human blood using 67857-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD42b Monoclonal antibody (67857-1-Ig)

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD42b Monoclonal antibody (67857-1-Ig)

    IHC staining of mouse spleen using 67857-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman peripheral blood platelets, human blood
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67857-1-Ig targets CD42b in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD42b fusion protein Ag31181
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated Molecular Weight 626 aa, 69 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
RRIDAB_2918615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Protocols

Product Specific Protocols
WB protocol for CD42b antibody 67857-1-IgDownload protocol
IHC protocol for CD42b antibody 67857-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols