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PPARGC1B Monoclonal antibody

PPARGC1B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D2E8

Cat no : 67821-1-Ig

Synonyms

ERRL1, PERC, PGC 1 beta, PGC 1(beta), PGC1, PGC1B, PPAR gamma coactivator 1 beta, PPARGC 1 beta, PPARGC1, PPARGC1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis using 67821-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of SH-SY5Y using 67821-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of pig brain using 67821-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of rat brain using 67821-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of mouse brain using 67821-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of HeLa using 67821-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PPARGC1B Monoclonal antibody (67821-1-Ig)

    WB analysis of MCF-7 using 67821-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67821-1-Ig targets PPARGC1B in WB, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPARGC1B fusion protein Ag28861
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 beta
Calculated Molecular Weight 1023 aa, 113 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC132971
Gene Symbol PPARGC1B
Gene ID (NCBI) 133522
RRIDAB_2918584
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPARGC1B antibody 67821-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Chem Biol Interact

N-acetylcysteine delayed cadmium-induced chronic kidney injury by activating the sirtuin 1-P53 signaling pathway

Authors - Wenxuan Dong
View Article
mouseWB

Sci Rep

Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice

Authors - Yusuke Komiya
View Article