• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MVK Monoclonal antibody

MVK Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F1D3

Cat no : 67820-1-Ig

Synonyms

FLJ96772, LRBP, mevalonate kinase, MK, MVK, MVLK

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using MVK Monoclonal antibody (67820-1-Ig)

    WB analysis using 67820-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67820-1-Ig (MVK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using MVK Monoclonal antibody (67820-1-Ig)

    WB analysis of U2OS using 67820-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67820-1-Ig (MVK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, U2OS cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67820-1-Ig targets MVK in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MVK fusion protein Ag28887
Full Name mevalonate kinase
Calculated Molecular Weight 396 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC016140
Gene Symbol MVK
Gene ID (NCBI) 4598
RRIDAB_2918583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

Protocols

Product Specific Protocols
WB protocol for MVK antibody 67820-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols