TUFM Monoclonal antibody
TUFM Monoclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A10D2
Cat no : 67802-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67802-1-Ig targets TUFM in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | TUFM fusion protein Ag24976 |
Full Name | Tu translation elongation factor, mitochondrial |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 48-50 kDa |
GenBank Accession Number | BC010041 |
Gene Symbol | TUFM |
Gene ID (NCBI) | 7284 |
RRID | AB_2918565 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protocols
Product Specific Protocols | |
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WB protocol for TUFM antibody 67802-1-Ig | Download protocol |
IHC protocol for TUFM antibody 67802-1-Ig | Download protocol |
IP protocol for TUFM antibody 67802-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (02-19-2023) | Works very Well for western blot
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