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RPS12 Monoclonal antibody

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3D1

Cat no : 67683-1-Ig

Synonyms

40S ribosomal protein S12, ribosomal protein S12, RPS12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis using 67683-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    WB analysis of NCI-H1299 using 67683-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human colon cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS12 Monoclonal antibody (67683-1-Ig)

    IHC staining of human breast cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS12 Monoclonal antibody (67683-1-Ig)

    IF Staining of HepG2 using 67683-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, NCI-H1299 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, PC-12cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67683-1-Ig targets RPS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPS12 fusion protein Ag9649
Full Name ribosomal protein S12
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC017321
Gene Symbol RPS12
Gene ID (NCBI) 6206
RRIDAB_2882876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

Protocols

Product Specific Protocols
WB protocol for RPS12 antibody 67683-1-IgDownload protocol
IHC protocol for RPS12 antibody 67683-1-IgDownload protocol
IF protocol for RPS12 antibody 67683-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols