Filter: AllWBIHCIF-PCytometric bead array

WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51282-1
Cytometric bead array standard curve of MP51282-1, FREQ Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67616-1-PBS. Detection antibody: 67616-2-PBS. Standard:Ag29808. Range: 1.563-100 ng/mL.

NCS1 Monoclonal antibody, PBS Only (Capture)

NCS1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 67616-1-PBS

Clone No.2F10C8

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67616-1-PBS targets NCS1 as part of a matched antibody pair:

MP51282-1: 67616-1-PBS capture and 67616-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	NCS1 fusion protein Ag29808 Predict reactive species
Full Name	frequenin homolog (Drosophila)
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	20 kDa
GenBank Accession Number	BC004856
Gene Symbol	NCS1
Gene ID (NCBI)	23413
RRID	AB_2882819
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P62166
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.