NCS1 Monoclonal antibody
NCS1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2F10C8
Cat no : 67616-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, U-87 MG cells, pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
67616-1-Ig targets NCS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Reactivity | Human, mouse, rat, pig |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NCS1 fusion protein Ag29808 |
Full Name | frequenin homolog (Drosophila) |
Calculated Molecular Weight | 22 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC004856 |
Gene Symbol | NCS1 |
Gene ID (NCBI) | 23413 |
RRID | AB_2882819 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NCS1 antibody 67616-1-Ig | Download protocol |
IHC protocol for NCS1 antibody 67616-1-Ig | Download protocol |
IF protocol for NCS1 antibody 67616-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons |