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CRX Monoclonal antibody

CRX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3C11

Cat no : 67555-1-Ig

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRX Monoclonal antibody (67555-1-Ig)

    WB analysis using 67555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CRX Monoclonal antibody (67555-1-Ig)

    WB analysis of Y79 using 67555-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CRX Monoclonal antibody (67555-1-Ig)

    IHC staining of mouse eye using 67555-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67555-1-Ig (CRX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat retina tissue, Y79 cells, mouse retina tissue, pig retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67555-1-Ig targets CRX in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRX fusion protein Ag30086
Full Name cone-rod homeobox
Calculated Molecular Weight 299 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC016664
Gene Symbol CRX
Gene ID (NCBI) 1406
RRIDAB_2882769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].

Protocols

Product Specific Protocols
WB protocol for CRX antibody 67555-1-IgDownload protocol
IHC protocol for CRX antibody 67555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols