PER2 (isoform 1) Monoclonal antibody

PER2 (isoform 1) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C3

Cat no : 67513-1-Ig

Synonyms

FASPS, hPER2, KIAA0347, PER2, period homolog 2 (Drosophila)



Tested Applications

Positive WB detected inHEK-293 cells, L02 cells, Y79 cells, BxPC-3 cells, K-562 cells, HL-60 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67513-1-Ig targets PER2 (isoform 1) in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PER2 (isoform 1) fusion protein Ag29489
Full Name period homolog 2 (Drosophila)
Calculated Molecular Weight 137 kDa
Observed Molecular Weight150~160 kDa
GenBank Accession NumberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
RRIDAB_2882734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).

Protocols

Product Specific Protocols
WB protocol for PER2 (isoform 1) antibody 67513-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Sci Total Environ

Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario

Authors - Fangnon Firmin Fangninou
humanWB

CNS Neurosci Ther

Cellular iron depletion enhances behavioral rhythm by limiting brain Per1 expression in mice

Authors - Qiong Wu
humanWB

Cancer Manag Res

Circadian Clock Protein PERIOD2 Suppresses the PI3K/Akt Pathway and Promotes Cisplatin Sensitivity in Ovarian Cancer.

Authors - Zhaoxia Wang
humanWB

J Immunol Res

Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.

Authors - Xiyan Tan
humanWB,IHC

Front Mol Biosci

Period2 is associated with immune cell infiltration and is a potential diagnostic and prognostic marker for hepatocellular carcinoma

Authors - Xiaolin Chen
mouseWB

J Cell Sci

The actin cytoskeleton-MRTF/SRF cascade transduces cellular physical niche cues to entrain circadian clock

Authors - Xuekai Xiong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Priya (Verified Customer) (01-05-2023)

I have been using this antibody for human keratinocytes, human cardiomyocytes and mouse tissues like liver and skin

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin