MAP1B Monoclonal antibody
MAP1B Monoclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1E6B9
Cat no : 67423-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67423-1-Ig targets MAP1B in IHC, IF-P, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MAP1B fusion protein Ag17709 |
| Full Name | microtubule-associated protein 1B |
| Calculated Molecular Weight | 2468 aa, 271 kDa |
| GenBank Accession Number | BC141853 |
| Gene Symbol | MAP1B |
| Gene ID (NCBI) | 4131 |
| RRID | AB_2918490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| IF protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| FC protocol for MAP1B antibody 67423-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
