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FUCA1 Monoclonal antibody

FUCA1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat no : 67251-1-Ig

Synonyms

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of HepG2 using 67251-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of K-562 using 67251-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of NIH/3T3 using 67251-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    WB analysis of HEK-293 using 67251-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IHC staining of human liver cancer using 67251-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IHC staining of human liver cancer using 67251-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of human liver cancer using 67251-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of human liver cancer using 67251-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FUCA1 Monoclonal antibody (67251-1-Ig)

    IF Staining of HepG2 using 67251-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67251-1-Ig targets FUCA1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FUCA1 fusion protein Ag9638
Full Name fucosidase, alpha-L- 1, tissue
Calculated Molecular Weight 461 aa, 53 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC017338
Gene Symbol FUCA1
Gene ID (NCBI) 2517
RRIDAB_2882528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Protocols

Product Specific Protocols
WB protocol for FUCA1 antibody 67251-1-IgDownload protocol
IHC protocol for FUCA1 antibody 67251-1-IgDownload protocol
IF protocol for FUCA1 antibody 67251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols