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APBB1 Monoclonal antibody

APBB1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B9G10

Cat no : 67077-1-Ig

Synonyms

APBB1, FE65, Protein Fe65, RIR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APBB1 Monoclonal antibody (67077-1-Ig)

    WB analysis using 67077-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse cerebellum using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse cerebellum using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse brain using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IHC staining of mouse brain using 67077-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using APBB1 Monoclonal antibody (67077-1-Ig)

    IF Staining of mouse brain using 67077-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, Pig brain, Rat brain, Mouse brain
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67077-1-Ig targets APBB1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen APBB1 fusion protein Ag27436
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Calculated Molecular Weight 708 aa, 77 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC010854
Gene Symbol APBB1
Gene ID (NCBI) 322
RRIDAB_2882385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).

Protocols

Product Specific Protocols
WB protocol for APBB1 antibody 67077-1-IgDownload protocol
IHC protocol for APBB1 antibody 67077-1-IgDownload protocol
IF protocol for APBB1 antibody 67077-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols