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PI3 Kinase p110 Alpha Monoclonal antibody
PI3 Kinase p110 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Pig, Rabbit, rabbit and More (6)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, RIP, ELISA
Conjugate
Unconjugated
CloneNo.
1F6A7
Cat no : 67071-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, pig brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 93 publications below |
| IHC | See 5 publications below |
| CoIP | See 1 publications below |
| RIP | See 1 publications below |
Product Information
67071-1-Ig targets PI3 Kinase p110 Alpha in WB, IHC, IF/ICC, FC (Intra), CoIP, RIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, rabbit samples.
| Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit, rabbit |
| Cited Reactivity | human, mouse, rat, monkey, bovine, fish |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PI3 Kinase p110 Alpha fusion protein Ag17392 |
| Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| Calculated Molecular Weight | 1068 aa, 124 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC113601 |
| Gene Symbol | PIK3CA |
| Gene ID (NCBI) | 5290 |
| RRID | AB_2882380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Alpha antibody 67071-1-Ig | Download protocol |
| IHC protocol for PI3 Kinase p110 Alpha antibody 67071-1-Ig | Download protocol |
| IF protocol for PI3 Kinase p110 Alpha antibody 67071-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis. | ||
Cancer Lett PI3K inhibitor impairs tumor progression and enhances sensitivity to anlotinib in anlotinib-resistant osteosarcoma. | ||
Oxid Med Cell Longev Transcriptional Patterns of Lower-Grade Glioma Patients with Distinct Ferroptosis Levels, Immunotherapy Response, and Temozolomide Sensitivity. | ||
Oxid Med Cell Longev Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Sci Total Environ PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Iru (Verified Customer) (02-18-2021) | Not sure what the band is, I saw a clear band in between 38 and 50KD.
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