CD97 Monoclonal antibody
CD97 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3F10C2
Cat no : 66972-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HepG2 cells, HL-60 cells, DC2.4 cells |
Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66972-1-Ig targets CD97 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD97 fusion protein Ag27991 |
Full Name | CD97 molecule |
Calculated Molecular Weight | 722 aa, 79 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC026690 |
Gene Symbol | CD97 |
Gene ID (NCBI) | 976 |
RRID | AB_2882293 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).
Protocols
Product Specific Protocols | |
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WB protocol for CD97 antibody 66972-1-Ig | Download protocol |
IF protocol for CD97 antibody 66972-1-Ig | Download protocol |
FC protocol for CD97 antibody 66972-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |