CD97 Monoclonal antibody, PBS Only

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  WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66972-1-Ig (same clone as 66972-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of DC2.4 using 66972-1-Ig (same clone as 66972-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66972-1-Ig (same clone as 66972-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

CD97 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66972-1-PBS

Clone No.3F10C2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ADGRE5, Adhesion G protein-coupled receptor E5, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5 subunit beta, CD97 antigen

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Product Information

66972-1-PBS targets CD97 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27991

  Product name: Recombinant human CD97 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 21-350 aa of BC026690

  Sequence: QDSRGCARWCPQNSSCVNATACRCNPGFSSFSEIITTPTETCDDINECATPSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDSKTSSAEVTIQNVIKLVDELMEAPGDVEALAPPVRHLIATQLLSNLEDIMRILAKSLPKGPFTYISPSNTELTLMIQERGDKNVTMGQSSARMKLNWAVAAGAEDPGPAVAGILSIQNMTTLLANASLNLHSKKQAELEEIYESSIRGVQLRRLS

• Full Name: CD97 molecule
• Calculated Molecular Weight: 722 aa, 79 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC026690
• Gene Symbol: CD97
• Gene ID (NCBI): 976
• RRID: AB_2882293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48960
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).