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ADK Monoclonal antibody

ADK Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G1A5

Cat no : 66929-1-Ig

Synonyms

EC:2.7.1.20, AK, adenosine kinase, Adenosine 5'-phosphotransferase, 1G1A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADK Monoclonal antibody (66929-1-Ig)

    WB analysis using 66929-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IHC staining of human liver cancer using 66929-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IHC staining of human liver cancer using 66929-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IF Staining of human liver cancer using 66929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IF Staining of human breast cancer using 66929-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using ADK Monoclonal antibody (66929-1-Ig)

    FC experiment of NIH/3T3 using 66929-1-Ig

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human breast cancer tissue
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66929-1-Ig targets ADK in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADK fusion protein Ag7466
Full Name adenosine kinase
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC003568
Gene Symbol ADK
Gene ID (NCBI) 132
RRIDAB_2882255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADK antibody 66929-1-IgDownload protocol
IHC protocol for ADK antibody 66929-1-IgDownload protocol
IF protocol for ADK antibody 66929-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Authors - Nicole M Sayles
View Article
human,mouseWB,IHC

Gastroenterology

Hepatocyte Adenosine Kinase Promotes Excessive Fat Deposition and Liver Inflammation

Authors - Honggui Li
View Article
mouseWB

EMBO J

Regulation of LRRK2 mRNA stability by ATIC and its substrate AICAR through ARE-mediated mRNA decay in Parkinson's disease

Authors - Qinfang Liu
View Article