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INF2 Monoclonal antibody

INF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4A11A7

Cat no : 66910-1-Ig

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using INF2 Monoclonal antibody (66910-1-Ig)

    WB analysis using 66910-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66910-1-Ig (INF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using INF2 Monoclonal antibody (66910-1-Ig)

    IHC staining of human kidney using 66910-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66910-1-Ig (INF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INF2 Monoclonal antibody (66910-1-Ig)

    IF Staining of HepG2 using 66910-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INF2 antibody (66910-1-Ig, Clone: 4A11A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A431 cells, PC-3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66910-1-Ig targets INF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen INF2 fusion protein Ag17043
Full Name inverted formin, FH2 and WH2 domain containing
Calculated Molecular Weight 1249 aa, 136 kDa
Observed Molecular Weight 150-170 kDa
GenBank Accession NumberBC008756
Gene Symbol INF2
Gene ID (NCBI) 64423
RRIDAB_2882237
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Protocols

Product Specific Protocols
WB protocol for INF2 antibody 66910-1-IgDownload protocol
IHC protocol for INF2 antibody 66910-1-IgDownload protocol
IF protocol for INF2 antibody 66910-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols