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TFPI Monoclonal antibody

TFPI Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B12F11

Cat no : 66842-1-Ig

Synonyms

EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using TFPI Monoclonal antibody (66842-1-Ig)

    WB analysis of human placenta using 66842-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TFPI Monoclonal antibody (66842-1-Ig)

    WB analysis of HepG2 using 66842-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66842-1-Ig targets TFPI in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TFPI fusion protein Ag14980
Full Name tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Calculated Molecular Weight251aa,29 kDa; 304aa,35 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC015514
Gene Symbol TFPI
Gene ID (NCBI) 7035
RRIDAB_2882183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Protocols

Product Specific Protocols
WB protocol for TFPI antibody 66842-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.

Authors - Ting Li
View Article