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PRDX3 Monoclonal antibody

PRDX3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7F7

Cat no : 66810-1-Ig

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDX3 Monoclonal antibody (66810-1-Ig)

    WB analysis using 66810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PRDX3 Monoclonal antibody (66810-1-Ig)

    WB analysis of HEK-293 using 66810-1-Ig

    WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66810-1-Ig targets PRDX3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityrat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PRDX3 fusion protein Ag1062
Full Name peroxiredoxin 3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
RRIDAB_2882153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRDX3 antibody 66810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Free Radic Biol Med

Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling.

Authors - Yanan Chen
View Article
ratWB

Ecotoxicol Environ Saf

Mechanism of KAT2A regulation of H3K36ac in manganese-induced oxidative damage to mitochondria in the nervous system and intervention by curcumin

Authors - Yan Liu
View Article