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- KD/KO Validated
FANCL Monoclonal antibody
FANCL Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
6E2B6
Cat no : 66639-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
Product Information
66639-1-Ig targets FANCL in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FANCL fusion protein Ag6192 |
| Full Name | Fanconi anemia, complementation group L |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 41-43 kDa |
| GenBank Accession Number | BC054517 |
| Gene Symbol | FANCL |
| Gene ID (NCBI) | 55120 |
| RRID | AB_2881998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fanconi anemia (FA) is a hereditary bone marrow failure syndrome associated with developmental defects and cancer predisposition. The FA nuclear core complex consists of FANCA, B, C, E, F, G, L, and M. FANCL is a RING-type E3 ubiquitin ligase that monoubiquitinates FANCD2 and FANCI, a key functional role facilitated by other members of the nuclear core complex (PMID: 23783032). FANCL plays an important role in the repair of cisplatin-induced DNA damage (PMID: 26385482) and participates directly in support of stem cell function (PMID: 23783032). FANCL can be detected as 40-43 kDa (full-length) and ~28 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCL antibody 66639-1-Ig | Download protocol |
| IF protocol for FANCL antibody 66639-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.
| ||
J Mol Biol Mitotic DNA synthesis in untransformed human cells preserves common fragile site stability via a FANCD2-driven mechanism that requires HELQ
| ||
Cell Rep FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination
| ||
Nat Commun Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma |
