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STK3 Monoclonal antibody

STK3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11A4

Cat no : 66637-1-Ig

Synonyms

KRS1, MST 2, MST2, STE20 like kinase MST2, STK3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using STK3 Monoclonal antibody (66637-1-Ig)

    WB analysis of HeLa using 66637-1-Ig

    WB result of STK3 antibody (66637-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK3 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using STK3 Monoclonal antibody (66637-1-Ig)

    WB analysis using 66637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66637-1-Ig (STK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using STK3 Monoclonal antibody (66637-1-Ig)

    IF Staining of NIH/3T3 using 66637-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using STK3 antibody (66637-1-Ig, Clone: 1C11A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66637-1-Ig targets STK3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STK3 fusion protein Ag2741
Full Name serine/threonine kinase 3 (STE20 homolog, yeast)
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 56-63 kDa
GenBank Accession NumberBC010640
Gene Symbol STK3
Gene ID (NCBI) 6788
RRIDAB_2881996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STK3 antibody 66637-1-IgDownload protocol
IF protocol for STK3 antibody 66637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Gen Subj

Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP

Authors - Ana Paula Zen Petisco Fiore
View Article
humanWB,IF

Oncogene

Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired kinase and cell growth suppressing activities

Authors - Ana Maria Rodrigues
View Article