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  • Featured Product
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FAF2 Monoclonal antibody

FAF2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H6D2

Cat no : 66629-1-Ig

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66629-1-Ig (FAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAF2 Monoclonal antibody (66629-1-Ig)

    WB analysis of HeLa using 66629-1-Ig

    WB result of FAF2 antibody (66629-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of human breast cancer using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAF2 Monoclonal antibody (66629-1-Ig)

    IHC staining of mouse brain using 66629-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66629-1-Ig (FAF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66629-1-Ig targets FAF2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAF2 fusion protein Ag21536
Full Name Fas associated factor family member 2
Calculated Molecular Weight 445 aa, 53 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
RRIDAB_2881989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

Protocols

Product Specific Protocols
WB protocol for FAF2 antibody 66629-1-IgDownload protocol
IHC protocol for FAF2 antibody 66629-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols