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CA3 Monoclonal antibody

CA3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat , Pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3C10A2

Cat no : 66608-1-Ig

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA3 Monoclonal antibody (66608-1-Ig)

    WB analysis using 66608-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IHC staining of mouse skeletal muscle using 66608-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IHC staining of mouse skeletal muscle using 66608-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IF Staining of mouse skeletal muscle using 66608-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inpig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66608-1-Ig targets CA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.

Tested Reactivity Human, Mouse , Rat , Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CA3 fusion protein Ag7513
Full Name carbonic anhydrase III, muscle specific
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC004897
Gene Symbol CA3
Gene ID (NCBI) 761
RRIDAB_2881968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Protocols

Product Specific Protocols
WB protocol for CA3 antibody 66608-1-IgDownload protocol
IHC protocol for CA3 antibody 66608-1-IgDownload protocol
IF protocol for CA3 antibody 66608-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols