• Featured Product
  • KD/KO Validated

ARC Monoclonal antibody

ARC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4B5

Cat no : 66550-1-Ig

Synonyms

ARC, ARC/ARG3.1, Arg3.1, KIAA0278



Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, Neuro-2a cells, pig brain tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66550-1-Ig targets ARC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, rabbit
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARC fusion protein Ag9445
Full Name activity-regulated cytoskeleton-associated protein
Calculated Molecular Weight 396 aa, 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC012321
Gene Symbol ARC
Gene ID (NCBI) 23237
RRIDAB_2881912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.

Protocols

Product Specific Protocols
WB protocol for ARC antibody 66550-1-IgDownload protocol
IHC protocol for ARC antibody 66550-1-IgDownload protocol
IF protocol for ARC antibody 66550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells.

Authors - Yu-Yuan Wang
  • KD Validated
human,ratWB,IF

Front Aging Neurosci

Methyltransferase-Like 3 Rescues the Amyloid-beta protein-Induced Reduction of Activity-Regulated Cytoskeleton Associated Protein Expression via YTHDF1-Dependent N6-Methyladenosine Modification.

Authors - Chenhaoyi Xu
mouseWB

eNeuro

Phosphorylation of CREB at Serine 142 and 143 Is Essential for Visual Cortex Plasticity.

Authors - Nisha S Pulimood
ratWB

Neurochem Res

Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model.

Authors - Yuriko Iwakura
ratWB

Neurotox Res

Inhibition of NLRP1-Dependent Pyroptosis Prevents Glycogen Synthase Kinase-3β Overactivation-Induced Hyperphosphorylated Tau in Rats

Authors - Xiangying Liu
ratWB

Biomed Pharmacother

Protective effect of 3-n-butylphthalide against intrastriatal injection of malonic acid-induced neurotoxicity and biochemical alteration in rats

Authors - Chunling Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Pegah (Verified Customer) (08-24-2023)

I used this antibody 1:1000 for detection of Arc in whole brain lysates immunoprecipitated for Arc (with an anti-rabbit antibody).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000