Filter: AllWBIHCIF-PIF-FroIF/ICCCytometric bead array

WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66496-1-Ig (same clone as 66496-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66496-1-Ig (same clone as 66496-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of U-251 using 66496-1-Ig (same clone as 66496-1-PBS)
U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 66496-1-Ig (same clone as 66496-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50295-3
Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.

Calretinin Monoclonal antibody, PBS Only (Detector)

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Cat No. 66496-1-PBS

Clone No.2D7A9

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, Cytometric bead array, Indirect ELISA

Synonyms

CALB2, 29 kDa calbindin, 2D7A9, CAB29, CAL2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66496-1-PBS targets Calretinin as part of a matched antibody pair:

MP50295-3: 66496-6-PBS capture and 66496-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2924 Product name: Recombinant human Calretinin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC015484 Sequence: MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLYRKDLEIVLCSEPPM Predict reactive species
Full Name	calbindin 2
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC015484
Gene Symbol	Calretinin
Gene ID (NCBI)	794
RRID	AB_2868480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P22676
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.