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  • Featured Product
  • KD/KO Validated

PADI2 Monoclonal antibody, PBS Only (Capture)

PADI2 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8C12

Cat no : 66386-1-PBS

Synonyms

PADI2 protein, PAD-H19, PAD2, PAD H19, KIAA0994

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of MCF-7 using 66386-1-Ig (same clone as 66386-1-PBS)

    WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of human skeletal muscle using 66386-1-Ig (same clone as 66386-1-PBS)

    human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of mouse brain using 66386-1-Ig (same clone as 66386-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of HeLa using 66386-1-Ig (same clone as 66386-1-PBS)

    WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of pig brain using 66386-1-Ig (same clone as 66386-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    WB analysis of rat brain using 66386-1-Ig (same clone as 66386-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using PADI2 Monoclonal antibody (66386-1-Ig)

    IF Staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50376-2

    Cytometric bead array standard curve of MP50376-2

    Cytometric bead array standard curve of MP50376-2, PADI2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66386-1-PBS. Detection antibody: 66386-3-PBS. Standard:Ag17612. Range: 0.781-100 ng/mL.

Product Information

66386-1-PBS targets PADI2 as part of a matched antibody pair:

MP50376-2: 66386-1-PBS capture and 66386-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PADI2 fusion protein Ag17612
Full Name peptidyl arginine deiminase, type II
Calculated Molecular Weight 665 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009701
Gene Symbol PADI2
Gene ID (NCBI) 11240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.