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  • Featured Product
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CLIC4 Monoclonal antibody

CLIC4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7F9

Cat no : 66343-1-Ig

Synonyms

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis of HeLa using 66343-1-Ig

    WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis using 66343-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    WB analysis of pig heart using 66343-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human heart using 66343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human heart using 66343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human kidney using 66343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Monoclonal antibody (66343-1-Ig)

    IHC staining of human kidney using 66343-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHuH-7 cells, pig heart tissue, HeLa cells, U2Os cells, pig kidney tissue, rabbit kidney tissue, rat kidney tissue, mouse kidney tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66343-1-Ig targets CLIC4 in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLIC4 fusion protein Ag2942
Full Name chloride intracellular channel 4
Calculated Molecular Weight 253 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC012444
Gene Symbol CLIC4
Gene ID (NCBI) 25932
RRIDAB_2881723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Protocols

Product Specific Protocols
WB protocol for CLIC4 antibody 66343-1-IgDownload protocol
IHC protocol for CLIC4 antibody 66343-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Oxid Med Cell Longev

Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.

Authors - Songhan Li
View Article