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FOXN1 Monoclonal antibody

FOXN1 Monoclonal Antibody for WB, IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

1D8B12

Cat no : 66337-1-Ig

Synonyms

Winged-helix transcription factor nude, WHN, RONU, Forkhead box protein N1, forkhead box N1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    WB analysis of Raji using 66337-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    WB analysis of Jurkat using 66337-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66337-1-Ig (FOXN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using FOXN1 Monoclonal antibody (66337-1-Ig)

    IHC staining of mouse thymus using 66337-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using FOXN1 Monoclonal antibody (66337-1-Ig)

    IHC staining of mouse thymus using 66337-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 66337-1-Ig (FOXN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using FOXN1 Monoclonal antibody (66337-1-Ig)

    FC experiment of A549 using 66337-1-Ig

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human FOXN1 (66337-1-Ig, Clone:1D8B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inmouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66337-1-Ig targets FOXN1 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOXN1 fusion protein Ag18207
Full Name forkhead box N1
Calculated Molecular Weight 648 aa, 69 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC146539
Gene Symbol FOXN1
Gene ID (NCBI) 8456
RRIDAB_2881717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality.

Protocols

Product Specific Protocols
WB protocol for FOXN1 antibody 66337-1-IgDownload protocol
IHC protocol for FOXN1 antibody 66337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols