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Peripherin Monoclonal antibody, PBS Only (Detector)

Peripherin Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat no : 66317-1-PBS

Synonyms

PRPH, NEF4, 1H10B3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis using 66317-1-Ig (same clone as 66317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of rat brain using 66317-1-Ig (same clone as 66317-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    WB analysis of rat colon using 66317-1-Ig (same clone as 66317-1-PBS)

    rat colon tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human brain using 66317-1-Ig (same clone as 66317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Monoclonal antibody (66317-1-Ig)

    IHC staining of human colon using 66317-1-Ig (same clone as 66317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using Peripherin Monoclonal antibody (66317-1-Ig)

    FC experiment of PC-12 using 66317-1-Ig (same clone as 66317-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66317-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51128-2

    Cytometric bead array standard curve of MP51128-2

    Cytometric bead array standard curve of MP51128-2, Peripherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66317-4-PBS. Detection antibody: 66317-1-PBS. Standard:Ag11116. Range: 0.781-100 ng/mL.

Product Information

66317-1-PBS targets Peripherin as part of a matched antibody pair:

MP51128-2: 66317-4-PBS capture and 66317-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11116
Full Name peripherin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC032703
Gene Symbol Peripherin
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.