Filter: AllWBSandwich ELISASample testIHCCytometric bead array

WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)
Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 66286-1-Ig (same clone as 66286-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP50642-3
Sandwich ELISA standard curve of MP50642-3, Human MYL3 Monoclonal Matched Antibody Pair - PBS only. 66286-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24535. 66286-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL
Sample test of MP50642-3
The mean MYL3 concentration was determined to be 23.06 ng/mL in mouse heart tissue extract based on a 6.9 mg/mL extract load and 3.2 ng/mL in mouse skeletal muscle tissue extract based on a 1.7 mg/mL extract load.
IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP50642-3
Cytometric bead array standard curve of MP50642-3, MYL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66286-1-PBS. Detection antibody: 66286-5-PBS. Standard:Ag24535. Range: 0.098-6.25 ng/mL.

MYL3 Monoclonal antibody, PBS Only (Capture)

MYL3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 66286-1-PBS

Clone No.2E4B2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66286-1-PBS targets MYL3 as part of a matched antibody pair:

MP50642-3: 66286-1-PBS capture and 66286-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	MYL3 fusion protein Ag24535 Predict reactive species
Full Name	myosin, light chain 3, alkali; ventricular, skeletal, slow
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC009790
Gene Symbol	MYL3
Gene ID (NCBI)	4634
RRID	AB_2881669
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P08590
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)