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NINJ2 Monoclonal antibody

NINJ2 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5C5B2

Cat no : 66202-1-Ig

Synonyms

Nerve injury induced protein 2, NINJ2, ninjurin 2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NINJ2 Monoclonal antibody (66202-1-Ig)

    IHC staining of human tonsillitis using 66202-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66202-1-Ig (NINJ2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NINJ2 Monoclonal antibody (66202-1-Ig)

    IHC staining of human tonsillitis using 66202-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66202-1-Ig (NINJ2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using NINJ2 Monoclonal antibody (66202-1-Ig)

    IF Staining of human tonsillitis using 66202-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NINJ2 antibody (66202-1-Ig, Clone: 5C5B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66202-1-Ig targets NINJ2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NINJ2 fusion protein Ag5343
Full Name ninjurin 2
Calculated Molecular Weight 142 aa, 16 kDa
GenBank Accession NumberBC057766
Gene Symbol NINJ2
Gene ID (NCBI) 4815
RRIDAB_2881593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)

Protocols

Product Specific Protocols
IHC protocol for NINJ2 antibody 66202-1-IgDownload protocol
IF protocol for NINJ2 antibody 66202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols