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  • Featured Product
  • KD/KO Validated

Cyclophilin B Monoclonal antibody

Cyclophilin B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

8H4F1

Cat no : 66047-1-Ig

Synonyms

PPIB, Peptidyl-prolyl cis-trans isomerase B, EC:5.2.1.8, CYP-S1, CYPB

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HeLa using 66047-1-Ig

    WB result of Cyclophilin B antibody (66047-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclophilin B transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis using 66047-1-Ig

    HeLa/Jurkat/ROS1728/PC-12/C6 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of RAW 264.7 using 66047-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HeLa using 66047-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of MCF-7 using 66047-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of HEK-293 using 66047-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    WB analysis of Jurkat using 66047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66047-1-Ig (Cyclophilin B antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66047-1-Ig (Cyclophilin B antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IHC staining of human pancreas cancer using 66047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66047-1-Ig(Cyclophilin B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    IF Staining of HeLa using 66047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin B antibody (66047-1-Ig, Clone: 8H4F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using Cyclophilin B Monoclonal antibody (66047-1-Ig)

    FC experiment of HeLa using 66047-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclophilin B (66047-1-Ig, Clone:8H4F1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66047-1-Ig targets Cyclophilin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclophilin B fusion protein Ag17957
Full Name peptidylprolyl isomerase B (cyclophilin B)
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight 19-24 kDa
GenBank Accession NumberBC032138
Gene Symbol Cyclophilin B
Gene ID (NCBI) 5479
RRIDAB_11042327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa).

Protocols

Product Specific Protocols
WB protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IHC protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
IF protocol for Cyclophilin B antibody 66047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Leon (Verified Customer) (07-06-2022)

Seems like a good antibody that detects cyclophilin B well and gave clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SH-SY5Y
Cyclophilin B Antibody Western Blot validation (1:5000 dilution) in SH-SY5Y (Cat no:66047-1-Ig)