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TPPII Monoclonal antibody

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5H6F11

Cat no : 66017-1-Ig

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Jurkat using 66017-1-Ig

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells.

  • Western Blot (WB) analysis of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HeLa using 66017-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HepG2 using 66017-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Raji using 66017-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of HeLa using 66017-1-Ig

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of MCF-7 using 66017-1-Ig

    Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Tested Applications

Positive WB detected inHela cells, Jurkat
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66017-1-Ig targets TPPII in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPII fusion protein Ag5296
Full Name tripeptidyl peptidase II
Calculated Molecular Weight 1249 aa, 138 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC039905
Gene Symbol TPPII
Gene ID (NCBI) 7174
RRIDAB_11044195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

Protocols

Product Specific Protocols
WB protocol for TPPII antibody 66017-1-IgDownload protocol
IHC protocol for TPPII antibody 66017-1-IgDownload protocol
IF protocol for TPPII antibody 66017-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression.

Authors - Xinyuan Lei
View Article
humanWB

J Immunol

Different Antigen-Processing Activities in Dendritic Cells, Macrophages, and Monocytes Lead to Uneven Production of HIV Epitopes and Affect CTL Recognition.

Authors - Jens Dinter
View Article