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  • Western Blot (WB) analysis of various lysates using NTRK1 Monoclonal antibody (60548-1-Ig)

    WB analysis using 60548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NK-92 cells using NTRK1 Monoclonal antibody (60548-1-Ig)

    WB analysis of NK-92 using 60548-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NTRK1 Monoclonal antibody (60548-1-Ig)

    WB analysis using 60548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60548-1-Ig (NTRK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

TrkA Monoclonal antibody

TrkA Monoclonal Antibody for WB, ELISA
Cat No. 60548-1-Ig
Clone No.3B11C7

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

NTRK1, Trk A, TRK, p140 TrkA, MTC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inKarpas-299 cells, NK-92 cells, SH-SY5Y cells, IMR-32 cells, TF-1 cells, K-562 cells, MOLT-4 cells, SK-N-SH cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60548-1-Ig targets TrkA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TrkA fusion protein Ag27985 Predict reactive species
Full Name neurotrophic tyrosine kinase, receptor, type 1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_001007792
Gene Symbol NTRK1
Gene ID (NCBI) 4914
RRIDAB_3670266
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NTRK1(Neurotrophic tyrosine kinase receptor 1) is located on chromosome 1q23.1 and is responsible for a rare genetic disorder, CIPA (congenital insensitivity to pain with anhidrosis) (PMID: 37907480). As a neurotrophin receptor, NTRK1 is abundantly expressed in human neuronal tissues and plays an integral role in the development and function of the human nervous system (PMID: 27843590). NTRK1 also regulates the survival and proliferation of cholinergic neurons (PMID: 20054812).

Protocols

Product Specific Protocols
WB protocol for TrkA antibody 60548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols