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CCT3 Monoclonal antibody

CCT3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

5F5A6

Cat no : 60264-1-Ig

Synonyms

CCT gamma, CCT3, CCTG, hTRiC5, PIG48, TCP 1 gamma, TRIC5

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CCT3 Monoclonal antibody (60264-1-Ig)

    WB analysis of COLO 320 using 60264-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60264-1-Ig (CCT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT3 Monoclonal antibody (60264-1-Ig)

    IHC staining of human liver cancer using 60264-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CCT3 Monoclonal antibody (60264-1-Ig)

    IHC staining of human liver cancer using 60264-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60264-1-Ig (CCT3 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CCT3 Monoclonal antibody (60264-1-Ig)

    IF Staining of HepG2 using 60264-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (60264-1-Ig, Clone: 5F5A6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60264-1-Ig targets CCT3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CCT3 fusion protein Ag0843
Full Name chaperonin containing TCP1, subunit 3 (gamma)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 56-60 kDa
GenBank Accession NumberBC006501
Gene Symbol CCT3
Gene ID (NCBI) 7203
RRIDAB_2881385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCT3 antibody 60264-1-IgDownload protocol
IHC protocol for CCT3 antibody 60264-1-IgDownload protocol
IF protocol for CCT3 antibody 60264-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Front Cell Dev Biol

Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins

Authors - Joshua Linnert
View Article