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CD23 Monoclonal antibody, PBS Only (Capture)

CD23 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

4C7C9

Cat no : 60208-2-PBS

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD23 Monoclonal antibody (60208-2-Ig)

    WB analysis of Raji using 60208-2-Ig (same clone as 60208-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD23 Monoclonal antibody (60208-2-Ig)

    IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50220-1

    Cytometric bead array standard curve of MP50220-1

    Cytometric bead array standard curve of MP50220-1, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-3-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50220-2

    Cytometric bead array standard curve of MP50220-2

    Cytometric bead array standard curve of MP50220-2, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3

    Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60208-2-PBS targets CD23 as part of a matched antibody pair:

MP50220-1: 60208-2-PBS capture and 60208-3-PBS detection (validated in Cytometric bead array)

MP50220-2: 60208-2-PBS capture and 60208-4-PBS detection (validated in Cytometric bead array)

MP50220-3: 60208-2-PBS capture and 60208-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated Molecular Weight 321 aa, 36 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC064417
Gene Symbol CD23
Gene ID (NCBI) 2208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.