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Septin 2 Monoclonal antibody
Septin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2F3B2
Cat no : 60075-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, Jurkat cells, fetal human brain tissue, human testis tissue, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, HSCT6 cells, ROS1728 cells, Neuro-2A cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE cells, K-562 cells |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IF | See 7 publications below |
| CoIP | See 1 publications below |
Product Information
60075-1-Ig targets Septin 2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Septin 2 fusion protein Ag1946 |
| Full Name | septin 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC014455 |
| Gene Symbol | Septin 2 |
| Gene ID (NCBI) | 4735 |
| RRID | AB_2187016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| IHC protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| IF protocol for Septin 2 antibody 60075-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. | ||
Cell Res Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly. | ||
Cell Rep Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts. | ||
Cytoskeleton (Hoboken) A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Amaury (Verified Customer) (03-29-2022) | I performed immunofluorescence on K562. It gave me very nice immunolabelling. I used ice-cold methanol to permeabilize the cells.
 |
FH Stephen (Verified Customer) (01-31-2019) | works fine for WB in HeLa cells 10ug of protein lysate loaded onto 10% NuPAGE gel run I in 1XMOPS buffer blot transferred onto nitrocelluloseAntibody used at 1:2000 incubated overnight at 4 degrees t in 5% milk PBST solution.
 |
