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  • Featured Product
  • KD/KO Validated

TDP-43 (human specific) Monoclonal antibody, PBS Only (Capture/Detector)

TDP-43 (human specific) Monoclonal Antibody for WB, IP, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6H6E12

Cat no : 60019-2-PBS

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43, TDP-43 (human specific)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    WB analysis using 60019-2-Ig (same clone as 60019-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50030-1

    Sandwich ELISA standard curve of MP50030-1

    Sandwich ELISA standard curve of MP50030-1, TDP-43 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66734-2-PBS. Detection antibody: 60019-2-PBS. Standard: Ag13119. Range: 6.25-400 ng/mL.

  • Sandwich ELISA standard curve of MP80000-1

    Sandwich ELISA standard curve of MP80000-1

    Sandwich ELISA standard curve ofMP80000-1, TDP-43 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60019-2-PBS. Detection antibody: 80001-1-PBS. Standard: Ag1833. Range: 78.1-5000 pg/mL.

  • Immunoprecipitation (IP) experiment of K-562 cells using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IP experiment of K-562 using 60019-2-Ig (same clone as 60019-2-PBS)

    IP result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug. This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human gliomas using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain(FTLD-U) tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human brain(FTLD-U) tissue using 60019-2-Ig (same clone as 60019-2-PBS)

    40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human pancreas cancer using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TDP-43 (human specific) Monoclonal antibody (60019-2-Ig)

    IHC staining of human pancreas cancer using 60019-2-Ig (same clone as 60019-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60019-2-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60019-2-PBS targets TDP-43 (human specific) as part of a matched antibody pair:

MP50030-1: 66734-2-PBS capture and 60019-2-PBS detection (validated in Sandwich ELISA)

MP80000-1: 60019-2-PBS capture and 80001-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.