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KNOP1 Polyclonal antibody

KNOP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30115-1-AP

Synonyms

101F10.1, C16orf88, FAM178A, Protein C16orf88, TSG118

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KNOP1 Polyclonal antibody (30115-1-AP)

    WB analysis using 30115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KNOP1 Polyclonal antibody (30115-1-AP)

    WB analysis using 30115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30115-1-AP (KNOP1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KNOP1 Polyclonal antibody (30115-1-AP)

    IF Staining of MCF-7 using 30115-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KNOP1 antibody (30115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inK-562 cells, U-251 cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30115-1-AP targets KNOP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KNOP1 fusion protein Ag32395
Full Name chromosome 16 open reading frame 88
Calculated Molecular Weight52 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_001012991.2
Gene Symbol KNOP1
Gene ID (NCBI) 400506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lysine-rich nucleolar protein 1 (KNOP1) is a nucleolar protein that interacts with zinc finger 106 protein. Also known as Tsg118, it was first reported in 1999 (3). KNOP1 is predominantly expressed in proliferating somatic cells and in male germ cells, and may therefore be involved in testicular development. (PMID: 37588747)

Protocols

Product Specific Protocols
WB protocol for KNOP1 antibody 30115-1-APDownload protocol
IF protocol for KNOP1 antibody 30115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols