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DNAH6 Polyclonal antibody

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30073-1-AP

Synonyms

Ciliary dynein heavy chain 6, DKFZp667K087, DNAH6, Dnahc6, DNHL1, Dynein heavy chain 6, axonemal, FLJ23529, FLJ37357, HL 2, HL2, KIAA1697

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAH6 Polyclonal antibody (30073-1-AP)

    WB analysis using 30073-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNAH6 Polyclonal antibody (30073-1-AP)

    IHC staining of mouse testis using 30073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DNAH6 Polyclonal antibody (30073-1-AP)

    IF Staining of hTERT-RPE1 using 30073-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30073-1-AP targets DNAH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAH6 fusion protein Ag32364
Full Name dynein, axonemal, heavy chain 6
Calculated Molecular Weight475 kDa
Observed Molecular Weight 475 kDa
GenBank Accession NumberBC104884
Gene Symbol DNAH6
Gene ID (NCBI) 1768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

Protocols

Product Specific Protocols
WB protocol for DNAH6 antibody 30073-1-APDownload protocol
IHC protocol for DNAH6 antibody 30073-1-APDownload protocol
IF protocol for DNAH6 antibody 30073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols